ELECTRON MICROSCOPE OBSERVATIONS ON FROZEN-DRIED CELLS
نویسندگان
چکیده
منابع مشابه
Electron Microscope Observations on Frozen-dried Cells
To explore the problem of artefacts which may be produced during usual fixation, dehydration, and embedding, the authors have examined pancreas, liver, and bone marrow frozen at about -180 degrees C., dried, at -55 to -60 degrees C., embedded in methacrylate, sectioned, and floated on a formol-alcohol mixture. By these treatments the labile structure of living cells can be fixed promptly and em...
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Relatively few malignant neoplasms have been described in amphibia. The one that has been studied most extensively is the Luck6 renal adenocarcinoma of the frog, Rana pipiens. This malignant tumor occurs in from two to four per cent of frogs of this species, collected in the Lake Champlain region of northern Vermont and adjoining areas of Quebec. In a series of papers Luck6 described its histop...
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An electron microscope study has been made of the structure of parietal cells in cats, dogs, and rats and of the cells lining the gastric glands of Bufo spinulosus. It is characteristic of all these cells to contain numerous vesicles about 0.05 to 0.3 micro in size. In the mammalian parietal cells an intracellular system of canaliculi is also observed, which is much more complex in the rat than...
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Small pieces of liver from rats subjected to different dietary regimes were fixed by freeze-drying, and postfixed by in vacuo heating and denaturation with alcohol. Specimens were digested with ribo- or deoxyribonuclease, and stained with gallocyanin-chromalum, azure II, the Feulgen procedure or alcoholic platinic tetrabromide. Some specimens were reserved as controls of the effects of enzyme t...
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A method is described by which single mitotic cells growing in tissue culture can be selected under the light microscope and then sectioned for electron-microscopic study. The method has been applied to mitotic cells in newt heart cultures and to monolayer cultures of human fibroblasts. The structure of the chromosomes and of the mitotic apparatus at various phases of mitosis are described and ...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1960
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.8.3.617